Efficient method of species identification and detection of sterlet’s hybrids (Acipenser ruthenus L.)
Abstract
The objective of work is to estimate the efficiency of using microsatellite markers of mitochondrial DNK for identifying species attribute and discovery of sterlet’s hybrids (Acipenser ruthenus L.) grown in fish farms of the Republic of Belarus.
Analysis of D-loop mitochondrial DHK with 41 bions of starlet spawners by means of species specific primers for sterlet (Ruf- length of DNK fragments-190 p.o.) and great sturgeon (Ruf- length of DNK fragments 374 p.o.) showed that the extracted and analyzed spawners’ mitochondrial DNK for 100% belong to sterlet. In PCR with a couple of primers species-specific for great sturgeon, accumulation of PCR product was not observed.
It was ascertained that among the spawners under study no sterlet hybrid forms are found.
Study of mitochondrial DNK represents the efficient method for species identification and detection of hybrids in sterlet spawners stock.
About the Authors
O. Koneva
Institute of Genetics and Cytology of NAS of Belarus
Belarus
Belarus
220027, Akademicheskaya Street, 27, Minsk.
E. Rovba
Institute of Genetics and Cytology of NAS of Belarus
Belarus
Belarus
220027, Akademicheskaya Street, 27, Minsk.
M. Lesjuk
Institute of Genetics and Cytology of NAS of Belarus
Belarus
Belarus
220027, Akademicheskaya Street, 27, Minsk.
A. Slukvin
Institute of Genetics and Cytology of NAS of Belarus
Belarus
Belarus
220027, Akademicheskaya Street, 27, Minsk.
References
1. Мюге Н.С. 2008. Полиморфизм контрольного региона митохондриальной ДНК восьми видов осетровых и разработка системы ДНК- идентификации видов / Н.С. Мюге, А.Е. Барминцева, С.М. Расторгуев, В.Н. Мюге, В.А. Барминцев // Генетика. – Т. 44, №7. – С. 913-919.
2. Барминцева А.Е. 2013. Использование микросателлитных локусов для установления видовой принадлежности осетровых (Acipenseridae) и выявления особей гибридного происхождения /А.Е. Барминцева, Н.С. Мюге // Генетика животных. – Т. 49, №9. – С. 1093-1105.
Review
For citations:
Koneva O., Rovba E., Lesjuk M., Slukvin A. Efficient method of species identification and detection of sterlet’s hybrids (Acipenser ruthenus L.). Belarus Fish Industry Problems. 2015;(31):168-177. (In Russ.)
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